The blot was probed with rabbit anti-phosphoEGFR and mouse anti-ERK2 followed by detection with IRDye 800CW Goat anti-Rabbit IgG ( P/N 926-32211) and IRDye 680RD Goat anti-Mouse IgG ( P/N 926-68070). Lysates were resolved by SDS-PAGE and transferred to nitrocellulose. A431 cells were treated with serial dilutions of EGF (6.3-200 ng/mL). Use Odyssey Western Blotting Kits for Multiplexing Dose response of EGFR phosphorylation in A431 cells. All kits include Intercept ® (PBS) Blocking Buffer or Intercept (TBS) Blocking Buffer. Kits are available with either 10 Immobilon ®-FL PVDF Membranes (0.45 μm, 10 cm x 10 cm) or 10 Odyssey Nitrocellulose Membranes (0.22 μm, 7 cm x 8.5 cm). Experiment with multiplex Western blotting for simultaneous two-color detectionĮach kit contains one IRDye ® 800CW dye-labeled secondary antibody for the 800 nm channel, plus an additional secondary antibody labeled with either IRDye 680RD or IRDye 680LT for the 700 nm channel.Use near-infrared fluorescence detection on the Odyssey Imaging Systems Antibodies Western Blotting Kits WesternSure Chemiluminescent Reagents Western Blot Incubation Boxes.Conveniently test LI-COR reagents and membranes for your Western blotting Black blot boxes are suitable for incubating near-infrared fluorescent and chemiluminescent Western blots or protein gels.Size: 7.2 cm x 4.9 cm x 3.0 cm Catalog No.Signal Total - ( Background x Area) Background: See Adaptive Background Subtraction for more information. Signal: Sum of the individual pixel intensity values (Total) for a band box (or lane box) minus the product of the background value and area. These sample kits allow you to obtain a smaller quantity of infrared reagents and corresponding membranes to perform quantitative Western blots without committing to a large quantity of each. Area: Number of pixels enclosed by a lane box or band box. Convenient Kits for Multiplex Western Blots RNAi studies in which a phosphate-buffered saline (PBS) buffering system or a Tris-buffered saline (TBS) is used. Black blot boxes are suitable for incubating near-infrared fluorescent and chemiluminescent Western blots or protein gels. Western blots, multiplex gels, IR and routine imaging of fluorescent and visible gels and blots. Kits include10 Odyssey Nitrocellulose Membranes (0.22 μm, 7 cm x 8.5 cm), and either Intercept ™ (TBS) Blocking Buffer or Intercept (PBS) Blocking Buffer.Odyssey Western Blotting Kits can be used for quantitative Western blots, In-Gel Westerns, or Highly sensitive for advanced chemiluminescent. The Western blotting kits provide enough secondary antibodies, blocking buffer, and membranes for 10 Western mini-blots.Įach kit contains one IRDye ® 800CW Dye-Labeled Secondary Antibody for the 800nm channel, plus an additional secondary antibody labeled with IRDye 680RD for the 700nm channel. Saving Protein Servicebio 1 Grid Antibody Uniform Polystyrene Sterile Plastic Incubation Box, Find Details about Licor Incubation Box, Western Blot Membrane.
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